Browse ArabiTag Libraries


ESTs in Library Library Name
(dbEST)
Description
670 Strasbourg-A
tissue=sliced leaves of A.thaliana ecotype columbia; clone_library=Strasbourg-A; Cloning vector: Lambda ZAPII; Physiological condition: leaves strips incubated 2/3/4 days in liquid culture medium. more
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985 Grenoble-B
tissue=Flower buds of A.thaliana ecotype columbia C24; clone_library=Grenoble-B; Cloning vector: Lambda ZAP. more
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286 Gif-SeedA+B
tissue=Green siliques of A.thaliana ecotype columbia; clone_library=Gif-SeedA+B; Cloning vector: Lambda ZAPII non-oriented. more
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7 Versailles-VD
In vitro-grown etiolated seedlings, 5 days old. more
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325 Ors-A AS Events Supported
1515 Versailles-VB AS Events Supported
124 Versailles-VC AS Events Supported
898 AC16H AS Events Supported
2 Grenoble-A
tissue=Flower buds of A.thaliana ecotype columbia C24. more
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99 AT-NHC
The AT-NHC library was made in Nam-Hai Chua's lab Rockefeller Univ. from whole plants. This cDNA library was made in Statagene's Lambda Zap using SmaI linkers and EcoRI-SmaI adaptors and cloned into the EcoRI site. more
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677 Lambda-PRL1
Lambda PRL1 is a cDNA library derived from equal quantities of 4 pools of mRNA. The mRNA sources were 1) 7 day germinated etiolated seedlings; 2) tissue culture grown roots; 3) staged plants half with 24 hour light cycle, half on 16 hr light, 8 hour more
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28024 Lambda-PRL2
Lambda PRL2 is a cDNA library derived from equal quantities of 4 pools of mRNA. The mRNA sources were 1) 7 day germinated etiolated seedlings; 2) tissue culture grown roots; 3) staged plants half with 24 hour light cycle, half on 16 hr light, 8 hour more
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121 Ra147.1
Physiological conditions: cycling cells one hour post-inoculation with X.campestris pv campestris strain 147 in the presence of cycloheximide 1 micromolar. more
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392 Gif-SeedA
Physiological condition: greenhouse plants. tissue_type: Green siliques more
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37 Strasbourg-FA
Physiological condition: leaves strips incubated 2/3/4 days in liquid culture medium. tissue_type: sliced leaves more
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116 Gif-SiliqueB
Physiological condition: greenhouse plants. tissue_type: Flowering tips more
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346 Perp-dry-seedA
tissue_type: Dry seeds more
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123 AC13D AS Events Supported
241 Ors-B
Physiological condition: nitrate treated liquid-cultured A.thaliana. tissue_type: roots more
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3 Arabidopsis thaliana M.W.Schena AS Events Supported
333 CD4-14
Using 5 ug of polyadenylated mRNA from 3 day-old Arabidopsis thaliana (Columbia) seedling hypocotyls as template and oligo d(t) as primer, first strand synthesis was catalyzed by Moloney murine leukemia virus reverse transcriptase (Pharmacia). Second more
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419 CD4-15
Using 5 ug of polyadenylated mRNA from 3 day-old Arabidopsis thaliana (Columbia) seedling hypocotyls as template and oligo d(t) as primer, first strand synthesis was catalyzed by Moloney murine leukemia virus reverse transcriptase (Pharmacia). Second more
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567 CD4-16
Using 5 ug of polyadenylated mRNA from 3 day-old Arabidopsis thaliana (Columbia) seedling hypocotyls as template and oligo d(t) as primer, first strand synthesis was catalyzed by Moloney murine leukemia virus reverse transcriptase (Pharmacia). Second more
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726 CD4-13
Using 5 ug of polyadenylated mRNA from 3 day-old Arabidopsis thaliana (Columbia) seedling hypocotyls as template and oligo d(t) as primer, first strand synthesis was catalyzed by Moloney murine leukemia virus reverse transcriptase (Pharmacia). Second more
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6 Arabidopsis thaliana mRNA (J.Fleck)
equal quantities of 4 mRNA pools: 7 day old germinated etiolated seeds; tissue culture grown roots; staged plants with 24h light cycle, half on 16h light; and aerial tissues (stems, flowers and siliques) of plants more
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184 NaCl-treated Arabidopsis subtraction library
Subtracted cDNA library from salt(NaCl)-treated whole plants more
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17710 Arabidopsis thaliana roots Columbia AS Events Supported
12403 Arabidopsis thaliana aboveground organs two to six-week old AS Events Supported
17 RAFL12
Subtraction Library. The sequence was obtained from samples subjected to cold-treated (1, 2, 5, 10, and 24 hr) more
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64 RAFL13
Subtraction Library. The sequence was obtained from samples subjected to dehydration-treated (1, 2, 5, 10, and 24 hr) more
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31884 RAFL14 AS Events Supported
14393 RAFL15 AS Events Supported
33996 RAFL16
dark-grown more
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14451 RAFL17
Subtraction Library. The sequence was obtained from samples subjected to dehydration-treated (1, 2, 5, 10 and 24 hr) and rehydration-treated (1, 2, 5, 10, and 24 hr after dehydration treatment) more
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1250 RAFL18
Subtraction Library. The sequence was obtained from samples subjected to cold-treated (1, 2, 5, 10, and 24 hr) more
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55599 RAFL19
Subtraction Library more
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40460 RAFL21
Subtraction Library. The sequence was obtained from samples subjected to various stress and plant hormones-treated more
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557 RAFL2 AS Events Supported
524 RAFL3
subjected to dehydration-treated(2hr) more
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2042 RAFL4
subjected to cold-treated(1,2,5,10,24 hr) more
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4241 RAFL5
subjected to dehydration-treated(1,2,5,10,24 hr) more
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8135 RAFL6
subjected to dehydration (1, 2, 5, 10, 24 hr) and cold (1, 2, 5, 10, 24 hr) treatments more
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22229 RAFL7
subjected to cold-treated (1, 2, 5, 10, 24 hr) more
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3375 RAFL8
subjected to dehydration-treated (1, 2, 5, 10, 24 hr) more
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29245 RAFL9
subjected to dehydration (1, 2, 5, 10, 24 hr) and cold (1, 2, 5, 10, 24 hr) treatments more
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2648 RAFL11
subjected to various treatments (dehydration, cold, high salt, ABA, heat and UV). Dark-grown plants more
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3345 Infected Arabidopsis Leaf
Mixed cDNA library of Arabidopsis and E. cichoracearum infected leaf from three weeks old Arabidopsis plants. Plants were harvested 3 days after infection and mRNA oligo dT selected. more
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4 Arabidopsis thaliana library (De Veylder L) AS Events Supported
49 Size-selected small cDNAs of Arabidopsis thaliana
cDNA names that include 'HR' are from the roots of hydroponically grown plants, names that include 'DS' are from developing seeds. All other clones are from two week old seedlings. more
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5 Arabidopsis thaliana Differential Display
Isolated from differential display of cDNAs from leaves of Arabidopsis thaliana wildtype after treatment with the fungal protein Nep1. more
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56 Methyl Jasmonate Treated Arabidopsis Forward-Subtracted
Clontech's PCR Suppression Subtractive Hybridization was used. Tester = plants sprayed with 50mM methyl jasmonate, and tissue harvested 1,8, 24 and 48h after treatment; driver = plants sprayed with water and tissue harvested 1,8, 24 and 48h. Cloned i more
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3 Methyl Jasmonate Treated Arabidopsis Reverse-Subtracted
Clontech's PCR Suppression Subtractive Hybridization was used. Tester = plants sprayed with water and tissue harvested 1,8, 24 and 48h; driver = plants sprayed with 50mM methyl jasmonate, and tissue harvested 1,8, 24 and 48h after treatment. Cloned i more
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46 Early Ovule Development Forward Subtracted Library
Clontech's PCR Suppression Subtractive Hybridization was used. Tester = ovaries collected within 24 hrs of pollination from wild type plants; driver = a male-sterile mutant. Cloned into Advantage PCR cloning vector (Clontech). more
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11 Virulent Peronospora parasitica Infected Arabidopsis
Clontech's PCR Suppression Subtractive Hybridization was used. Tester = plants sprayed with water and tissue harvested 1,3 and 5 days; driver = plants sprayed with spores of Peronospora parasitica strain Ahco, and tissue harvested 1,3 and 5 days afte more
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2 Avirulent Peronospora parasitica Infected Reverse Subtracted
Clontech's PCR Suppression Subtractive Hybridization was used. Tester = plants sprayed with water and tissue harvested 12h, 1, 2 and 3 days.; driver = plants sprayed with spores of Peronospora parasitica strain Emwa, and tissue harvested 12h, 1, 2 an more
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246 Virulent Peronospora parasitica Infected Arabidopsis
Clontech's PCR Suppression Subtractive Hybridization was used. Tester = plants sprayed with spores of Peronospora parasitica strain Ahco, and tissue harvested 1,3 and 5 days after infection ; driver = plants sprayed with water and tissue harvested 1, more
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110 Avirulent Peronospora parasitica Infected Forward Subtracted
Clontech's PCR Suppression Subtractive Hybridization was used. Tester = plants sprayed with spores of Peronospora parasitica strain Emwa, and tissue harvested 12h, 1, 2 and 3 days after infection ; driver = plants sprayed with water and tissue harves more
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145 Arabidopsis Acute Ozone pooled time-points
Clontech's PCR Suppression Subtractive Hybridization was used. Tester = plants exposed to 0.4 ul/L of ozone for 6 hours and tissue harvested 3, 6, 9,12 and 24h after treatment ; driver = plants exposed to clean air and tissue harvested 3, 6, 9 12 and more
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130 Arabidopsis Chronic Ozone Forward-Subtracted Library
PCR Suppression Subtractive Hybridization library (Clontech PCR-Select). 'Tester' = RNA pooled from leaf tissue of plants exposed to 3, 6, and 9 days of ozone at 0.15 ul/L for 6 h/d. 'Driver' = leaf tissue from control plants maintained in clean air. more
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110 Arabidopsis Salicylic Acid Subtracted Library
A cDNA library enriched for genes differentially expressed during activation of systemic acquired resistance (in response to salicylic acid treatment) was constructed using CLONTECH PCR-Select cDNA Subtraction Kit (CLONTECH Laboratories, Inc.). 'Test more
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235 Arabidopsis avirulent Pseudomonas syringae subtracted
A cDNA library enriched for Arabidopsis genes differentially expressed during activation of hypersensitive resistance (in response to infection by P. syringae pv. tomato DC3000 expressing avrRpm1) was constructed using CLONTECH PCR-Select cDNA Subtra more
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146 Arabidopsis virulent Pseudomonas syringae subtracted library
A cDNA library enriched for Arabidopsis genes differentially expressed during disease (in response to infection by P. syringae pv. tomato DC3000) was constructed using CLONTECH PCR-Select cDNA Subtraction Kit (CLONTECH Laboratories, Inc.). 'Tester'= more
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3551 MPIZ-ADIS-019
RNA from cellculture (At7) 5 days after inoculation treated with 0.002 mM cycloheximid for 2 h in the dark. Sequence submission managed by RZPD/GABI-Primary database: http://gabi.rzpd.de. This clone is available from RZPD; contact RZPD (clone@rzpd.de more
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1875 MPIZ-ADIS-027
cDNA library from Arabidopsis thaliana, accession Eifel-2; ten week old total plants grown under long-day conditions in soil, whole adult plants were treated for 24 hours with different stresses, (1) at 4M-0 C in the dark, (2), at 37 Grad C in the da more
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1627 MPIZ-ADIS-014
cDNA library from Arabidopsis thaliana, accession Cvi; six weeks old total plants grown under long-day conditions in soil, whole adult plants were treated for 24 hours with different stresses, (1) at 4 Grad C in the dark, (2), at 37 Grad C in the dar more
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1664 MPIZ-ADIS-013
RNA from total, adult, 6 weeks old Arabidopsis thaliana (accession Nd-1) plants, grown in the greenhouse under long day conditions in soil treated for 24 hours with different stresses, (1) at 4 Grad C in the dark, (2), at 37 Grad C in the dark, (3) l more
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1099 MPIZ-ADIS-012
cDNA library from Arabidopsis thaliana, accession Landsberg erecta; six weeks old total plants grown under long-day conditions in soil, whole adult plants were treated for 24 hours with different stresses, (1) at 4M-0 C in the dark, (2), at 37 Grad C more
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2499 MPIZ-ADIS-008
cDNA library from Arabidopsis thaliana, accession C24; seedling; Lambda ZAPII phage library was made at the Max-Planck-Institute of Molecular Plant Physiology, Golm, Germany and mass-excised at the Max-Planck-Institute for Plant Breeding Research, Co more
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1198 MPIZ-ADIS-035
cDNA library from Arabidopsis thaliana, accession Achkarren-2; inflorescences from flower buds to young siliques; library was made at the Max-Planck-Institute for Plant Breeding Research, Cologne, Germany; cloning sites SalI-NotI, primer sites and or more
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79 Cloned RT-PCR products of Arabidopsis thaliana
Gene specific primers used to amplify gene fragments from 1st strand cDNA more
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6164 Arabidopsis Leaf Senescence Library
Senescent rosette leaves #5 and #6 (counted from the bottom) were harvested and immediately frozen in liquid N2. The leaves were visibly yellow excepted for the leaf base areas that were still greenish. more
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2 Lambda-PRL2 (P.Kapranov)
PRL2 cDNA library, MSU-DOE Plant Research Laboratory, Michigan State University; Newman,T. et al., Plant Physiology 106: 1241-1255 (1994) more
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20 pi mRNA differential display clones
cDNA fragments from mRNA differential display of the pistillata (pi) floral homeotic mutant inserted in either orientation into pGEM-T vector (Promega). more
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201 CD4-6
Flower cDNA library, D. Weigel and associates (reference 217). Library data: RNA source: Landsberg erecta inflorescence with predominately younger flower buds. Features: made using Stratagene UniZap kit directionally cloned; 5' at EcoRI 3' at XhoI si more
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1 Arabidopsis thaliana 2 week old shoots AS Events Supported
28 Arabidopsis thaliana cell suspension
cell suspension; isolated with polyclonal antibodies against total protein from either plasma membrane or tonoplast more
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38 YAC clone CIC7E11 region-specific cDNA AS Events Supported
75 YAC clone CIC8B11 region-specific cDNA AS Events Supported
4 Hairy root culture
mRNA was prepared from prepared using polyA+ mRNA isolated from Arabidopsis hairy root cultures (ecotype C24) induced with Agrobacterium rhizogenes. The roots were treated with low-oxygen for 4 hours in liquid Murashige and Skoog (MS) medium, incubat more
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1559 MPIZ-ADIS-066
cDNA library from Arabidopsis thaliana, accession Wassilewskija-0; roots from three weeks old plants grown on MS-plates at 26M-0C with 16 hours light/day; library was made at the Max-Planck-Institute for Plant Breeding Research, Cologne, Germany; clo more
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1587 Arabidopsis ag-1 35S:AG-GR forward subtraction library
Subtractive library constructed using the CLONTECH PCR-select cDNA subtraction kit. Transgenic plants homozygous for the ag-1 mutation and carrying a 35S:AG-GR construct were generated. RNA was isolated from inflorescences with floral meristems and u more
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12754 Arabidopsis thaliana green siliques Columbia AS Events Supported
4504 AtM1
About 1 week after bolting, cDNA synthesis using SuperscriptTM-system (Invitrogen) with an oligo(dT)-primer containing NotI restriction site and a SalI adapter. The main library (plate numbers begin with 1) of 38,000 clones was rearrayed into the sub more
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84 Arabidopsis thaliana aerial vegetative tissues 4-weeks old
ecotype: Columbia 0 ; country: France more
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2417 Arabidopsis thaliana Adult vegetative tissue Col-0 AS Events Supported
2317 Arabidopsis thaliana Hormone Treated Callus Col-0 AS Events Supported
927 Arabidopsis thaliana Silique Col-0 AS Events Supported
1737 Arabidopsis thaliana Flowers and buds Col-0 AS Events Supported
342 Arabidopsis RT-PCR Products (CSHL)
DNA was extracted from Arabidopsis thaliana whole plant tissue, provided by members of Rob Martienssen's lab, using TRIZOL. Primers were designed in Hypothetical genes and un-annotated regions in Arabidopsis that are conserved in Brassica oleracea lo more
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35 RIKEN-PMB-FL1
RIKEN-PMB-FL1 more
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21 FL5 AS Events Supported
5 FL3 AS Events Supported
6 FL2 AS Events Supported
3366 Arabidopsis thaliana above-ground organ two to six-week old AS Events Supported
1161 Arabidopsis thaliana liquid-cultured seedlings Columbia AS Events Supported
27 cDNA-AFLP germinating seeds
cDNA fragments of genes expressed during seed germination from cDNA-AFLP experiment were cloned in pGEM-T vector more
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301 Arabidopsis Gateway cDNA Library
To clone DNA sequences for functional analysis and expression in multiple systems more
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34 Arabidopsis CaMs library AS Events Supported
49 Arabidopsis pSMASH Library
The plasmid vector, pSMASH, is a yeast expression vector derived from pGAD424 (Clontech). It was designed for selective cloning of the genes encoding secreted and membrane proteins. Briefly, it contains a EcoRI-NotI subcloning sites followed by a Kex more
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6 Arabidopsis thaliana library (Josefsson LG) AS Events Supported
27 Cloned cDNAs of Arabidopsis thaliana generated by mRNA
Templates for the differential-display RT-PCR were reverse-transcribed from total cellular RNA extracted (1) from excised feeding sites of roots with parasitic nematodes attached to it and (2) from adjacent root pieces of the same size supplemented w more
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1 Arabidopsis thaliana library (Shinozaki K) AS Events Supported
73 Arabidopsis thaliana library (Motohashi R) AS Events Supported
43241 CERES-149 AS Events Supported
632 CERES-AN56 AS Events Supported
72749 CERES-148 AS Events Supported
12465 CERES-AL45 AS Events Supported
15692 CERES-AL46 AS Events Supported
16401 CERES-AN65 AS Events Supported
32308 CERES-147 AS Events Supported
64 CERES-AL25 AS Events Supported
960 CERES-130 AS Events Supported
90 CERES-AL36 AS Events Supported
1274 CERES-AN AS Events Supported
217 CERES-AN10 AS Events Supported
1080 CERES-AS12 AS Events Supported
174 CERES-AN67 AS Events Supported
1400 CERES-AU1 AS Events Supported
92 CERES-AL15 AS Events Supported
294 CERES-AL6 AS Events Supported
957 CERES-131 AS Events Supported
797 CERES-129 AS Events Supported
136 CERES-AB1 AS Events Supported
150 CERES-AL5 AS Events Supported
230 CERES-AL16 AS Events Supported
299 CERES-AN55 AS Events Supported
28 CERES-AL35 AS Events Supported
13 CERES-AD65 AS Events Supported
7 CERES-AL3 AS Events Supported
15 Arabidopsis thaliana RT-PCR products
Direct sequencing of Arabidopsis thaliana RT-PCR products more
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80 MPIZ-ADIS-065a
In the context of the EU-funded project REGIA (QLG-CT11999-00876, coordinator Javier Paz-Ares), a set of transcription factor ORFs was generated. The ORFs were produced in a decentralized way in the labs of the participants. Most of the ORFs were gen more
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106 MPIZ-ADIS-065g
In the context of the GABI-TFs project (PI: Bernd Weisshaar; GABI-TFs was a part of the German plant genomics program GABI, FKZ 0312276N), a set of transcription factor ORFs was generated. The ORFs were generated by RT-PCR using cDNA from various A. more
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15 MPIZ-ADIS-065b
In the context of the EU-funded project REGIA (QLG-CT11999-00876, coordinator Javier Paz-Ares), a set of transcription factor ORFs was generated. The ORFs were produced in a decentralized way in the labs of the participants. Most of the ORFs were gen more
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12 MPIZ-ADIS-065c
In the context of the EU-funded project REGIA (QLG-CT11999-00876, coordinator Javier Paz-Ares), a set of transcription factor ORFs was generated. The ORFs were produced in a decentralized way in the labs of the participants. Most of the ORFs were gen more
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1828 MPIZ-ADIS-065d
In the context of the EU-funded project REGIA (QLG-CT11999-00876, coordinator Javier Paz-Ares), a set of transcription factor ORFs was generated. The ORFs were produced in a decentralized way in the labs of the participants. Most of the ORFs were gen more
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2 MPIZ-ADIS-065e
In the context of the EU-funded project REGIA (QLG-CT11999-00876, coordinator Javier Paz-Ares), a set of transcription factor ORFs was generated. The ORFs were produced in a decentralized way in the labs of the participants. Most of the ORFs were gen more
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168 MPIZ-ADIS-065f
In the context of the EU-funded project REGIA (QLG-CT11999-00876, coordinator Javier Paz-Ares), a set of transcription factor ORFs was generated. The ORFs were produced in a decentralized way in the labs of the participants. Most of the ORFs were gen more
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96 Aerial tissue from 28-day plants Columbia
cDNA was reverse transcribed and amplified by PCR. The primer sequences are the first 20 bp at each end. more
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143 Aerial tissue from 28-day plants Landsberg erecta
cDNA was reverse transcribed and amplified by PCR. The primer sequences are the first 20 bp at each end. more
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1 Arabidopsis thaliana 3' RACE
BD Biosciences 3' RACE kit primed with gene specific primer and 3'CDS primer A and UPM primers more
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1 Arabidopsis thaliana 5' RACE
BD Biosciences 5' RACE kit primed with gene specific primer and 5'CDS, SMARTII A oligo and UPM primers more
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1 Arabidopsis thaliana library (Tan K) AS Events Supported
4 Arabidopsis thaliana immature siliques
This sequence is obtained from a PCR product generated by differential display method. cDNA prepared from immature siliques (10 days after flowering) was used as the template more
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16 Arabidopsis thaliana germinating seeds AS Events Supported
3022 A. thaliana, Ohio State clone set
cDNA library was made from selected clones from the Arabidopsis thaliana Ohio State clone set. more
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367 A. thaliana, Columbia Col-0, inflorescence-1
cDNA library was derived from untreated inflorescence tissue from Arabidopsis thaliana, Columbia Col-0, at 4 - 7 weeks. Plants were grown in 1:1:1 peat moss/vermiculite/perlite soil at 22 deg. C +/- 3 deg. C under constant light, and watered with fer more
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692 A. thaliana, Columbia Col-0, inflorescence-2
cDNA library was derived from untreated inflorescence tissue from Arabidopsis thaliana, Columbia Col-0, at 4 - 7 weeks. Plants were grown in 1:1:1 peat moss/vermiculite/perlite soil at 22 deg. C +/- 3 deg. C under constant light, and watered with fer more
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562 A. thaliana, Columbia Col-0, root-1
cDNA library was derived from untreated root tissue from Arabidopsis thaliana, Columbia Col-0, at 4 - 7 weeks. Plants were grown in 1:1:1 peat moss/vermiculite/perlite soil at 22 deg. C +/- 3 deg. C under constant light, and watered with fertilizer. more
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676 A. thaliana, Columbia Col-0, root-2
cDNA library was derived from untreated root tissue from Arabidopsis thaliana, Columbia Col-0, at 4 - 7 weeks. Plants were grown in 1:1:1 peat moss/vermiculite/perlite soil at 22 deg. C +/- 3 deg. C under constant light, and watered with fertilizer. more
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640 A. thaliana, Columbia Col-0, rosette-1
cDNA library was derived from untreated rosette tissue from Arabidopsis thaliana, Columbia Col-0, at 4 - 7 weeks. Plants were grown in 1:1:1 peat moss/vermiculite/perlite soil at 22 deg. C +/- 3 deg. C under constant light, and watered with fertilize more
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897 A. thaliana, Columbia Col-0, rosette-2
cDNA library was derived from untreated rosette tissue from Arabidopsis thaliana, Columbia Col-0, at 4 - 7 weeks. Plants were grown in 1:1:1 peat moss/vermiculite/perlite soil at 22 deg. C +/- 3 deg. C under constant light, and watered with fertilize more
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814 A. thaliana, Columbia Col-0, rosette-3
cDNA library was derived from untreated rosette tissue from Arabidopsis thaliana, Columbia Col-0, at 4 - 7 weeks. Plants were grown in 1:1:1 peat moss/vermiculite/perlite soil at 22 deg. C +/- 3 deg. C under constant light, and watered with fertilize more
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272 A. thaliana, mixed source
This sequence was obtained from a clone generated with a PCR product of the target gene. more
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44 Lambda ZipLox designated PRL2 AS Events Supported
5 Arabidopsis thaliana Columbia AS Events Supported
17 Arabidopsis thaliana Above-ground organ from two to six-week AS Events Supported
178 Arabidopsis thaliana Col0 seed AS Events Supported
1 Arabidopsis thaliana Col met1-1 5' RACE
BD Biosciences 5' RACE kit primed with gene specific primer and 5'CDS, SMARTII A oligo and UPM primers more
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5 Arabidopsis thaliana Col ddm1-1 5' RACE
BD Biosciences 5' RACE kit primed with gene specific primer and 5'CDS, SMARTII A oligo and UPM primers more
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111302 pooled cDNA populations
Arabidopsis roots, inflorescence, callus, young seedlings and Arabidopsis treated with cold, heat, salt, 2,4-D, hydrogen peroxide, UV, IAA, Xanthomonas and Pseudomonas. more
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2 Arabidopsis thaliana TDNA insertion lines PCR
A Sequence-Indexed Library of Insertion Mutations in the Arabidopsis Genome. mRNA (EST) was obtained from transgenic line with artificial constitutive expression of 1st exon of At1g12860 gene. more
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1 Arabidopsis thaliana X-line TDNA insertion lines PCR
A Sequence-Indexed Library of Insertion Mutations in the Arabidopsis Genome more
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1 Arabidopsis thaliana O-line TDNA insertion lines PCR
A Sequence-Indexed Library of Insertion Mutations in the Arabidopsis Genome more
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541852 8-day Arabidopsis seedlings, aerial tissues
Arabidopsis seedlings grown on soil 8-days in continuous light at 20 C. RNA was extracted from aerial tissues and cDNA prepared from mRNA after oligodT priming. more
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1 Arabidopsis thaliana E9-line mRNA AS Events Supported
249438 Arabidopsis ovule high throughput cDNA library
Ovules were microdissected from stage14 flowers. 0.5 ug of RNA was reverse transcribed and PCR amplified for 15 cycles using the BD SMART protocol (Clontech). cDNAs were fragmented and sequenced by the 454 corporation. more
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97 Arabidopsis thaliana col-0 suppression subtraction cDNA AS Events Supported
462 Arabidopsis thaliana Col-0 RACE (RWM_NL_2007)
RACE-PCR was performed with the Marathon(TM) cDNA Amplification Kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. Total RNA was extracted from the leaves of about four-week old wild-type Arabidopsis Col-0 plants using t more
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2 Direct sequencing of RT-PCR products of Arabidopsis thaliana
Gene specific primers used to amplify gene fragments from 1st strand cDNA more
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1007 AA
20 h 200mM NaCl more
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642 AB
200mM NaCl more
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333 AC
20 h 150mM NaCl more
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5827 Arabidopsis thaliana flower buds Columbia AS Events Supported
3 Arabidopsis thaliana Col-0 RACE seven-day old seedling
RACE-PCR was performed with the Marathon(TM) cDNA Amplification Kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. Total RNA was extracted from the seven-day old seedling of Arabidopsis Col-0 plants using the TRIzol proc more
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11237 Arabidopsis developing seed AS Events Supported
9 Arabidopsis thaliana early developing seeds Library
mRNA derived from early developing seeds (heart stage embryo). cDNA library constructed using the SMART cDNA library technology according to the instructions of the manufacturer (CLONTECH). more
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171 Arabidopsis Acute Ozone Forward-Subtracted Library
PCR Suppression Subtractive Hybridization library (Clontech PCR-Select). 'Tester' = leaf tissue from plants exposed to 1 hr 0.4 ul/L ozone. 'Driver' = leaf tissue from control plants maintained in clean air. Cloned into pCR2.1TOPO vector (Invitrogen) more
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27 Arabidopsis Acute Ozone Reverse-Subtracted Library
PCR Suppression Subtractive Hybridization library (Clontech PCR-Select). 'Tester' = leaf tissue from plants exposed to 1 hr 0.4 ul/L ozone. 'Driver' = leaf tissue from control plants maintained in clean air. Cloned into pT-Adv vector (Clontech). more
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623 A AS Events Supported
551 B AS Events Supported
151 AD A. thaliana (Col-0 gl1) library enriched for salt-induced
AD Arabidopsis thaliana library enriched for salt-induced transcripts from wildtype (Col-0 gl1) 10-14 day-old seedlings treated with 160 mM NaCl for 4 hours. more
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571 AD A. thaliana (Col-0 gl1) subtracted library enriched for
AD Arabidopsis thaliana subtracted library enriched for salt-induced transcripts from wildtype (Col-0 gl1) 10-14 day-old seedlings treated with 160 mM NaCl for hours. more
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1 FL6 AS Events Supported